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P3. DOG-1 immunoreactivity: A single-institution experience in Romania

Simona Gurzu, Attila Kovecsi, Ioan Jung

Department of Pathology, University of Medicine and Pharmacy, Tirgu-Mures, Romania

Background: Although DOG-1 is considered a diagnostic marker for gastrointestinal stromal tumors (GIST), there are several other normal or tumor tissues that can display DOG-1 immunoreactivity. We present our experience in field of DOG-1 immunostain.

Methods: Immunohistochemical stains using DOG-1 antibody (Novocastra, Newcastle-upon-Tyne, UK) were performed in several normal tissues, 10 GISTs, 75 soft tissue tumors (STT), 55 cutaneous - and 25 digestive tumors. The clone K9, citrate incubation for 60 minutes and a concentration of 1:50 were used.

Results: DOG-1 positivity was showed by 8/10 GISTs. It was inconstantly expressed by lipoma (2/5 cases), pleomorphic and myxoid-type liposarcoma (6/36), dermatofibrosarcoma (1/5), leiomyosarcoma (5/13), malignant fibrous histiocytoma (1/3), basal- (1/17) and squamous cell carcinoma (8/38). Negativity was noted for atypical lipomatous tumor, granular cell tumor, dedifferentiated liposarcoma, fibrosarcoma, colorectal and gastric carcinomas, neuroendocrine tumors, mucinous cystic neoplasms of the pancreas, and hepatocellular carcinoma. From normal tissues, renal tubes, mature adipocytes, and eccrine glands were DOG-1 positive, whereas gastrointestinal tract mucosa and pancreatic parenchyma were negative.

Conclusions: DOG-1 positivity is an indicator for diagnosis of GISTs but the final diagnosis should take into account its correlation with other specific markers such c-KIT and CD34. The research was founded by project UMFTGM-PO-CC-02-F01-19/2014.

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